![]() More than 83% of diagnosed cases of diabetes are Type 2. According to the World Health Organization, over 50% of the population of the United States, Europe, and Australia, and low- to moderate-income countries such as India, Russia, Mexico, and Egypt are overweight or obese and at risk for developing diabetes. The obesity epidemic is a main contributor to the projected rise in these numbers. Approximately 1.5 million people annually are diagnosed with diabetes nationwide, with annual economic costs estimated at approximately $98 billion. While Type 1 diabetes is known to have a genetic basis, the underlying causes of Type 2 and gestational diabetes include both genetic and environmental (behavioral) factors.īased on Centers for Disease Control and Preventions’s national health interview survey statistics, some 7% of the US population has diabetes and of these approximately 30% are undiagnosed. The three main types of human diabetes are Type 1, Type 2, and gestational. The diluents kit allows researchers to choose the appropriate diluents kit for diluting samples, standards, and controls.The reagent kit includes an assay buffer, wash buffer, streptavidin-PE, 96-well filter plate, sealing tape, and panel-specific instructions required to prepare assays and acquire data.The singleplex assays (adiponectin and adipsin) may be mixed together to form a 2-plex assay. A premixed 12-plex of analytes (C-peptide, ghrelin, GIP, and visfatin) that includes GLP-1, glucagon, IL6, insulin, leptin, PAi-1, resistin, TNF-work with magnetic beads, preblended detection antibodies, standards, and controls.The system integrates assay kits, software, calibration and validation tools, and instrumentation. The suspension-array system is built around three core technologies: the family of fluorescently dyed microspheres (beads) from Luminex Corp a flow cytometer with two lasers and associated optics to measure biochemical reactions occurring on the surface of the microspheres and a high-speed digital signal processor to manage the fluorescent output. The microplate platform allows the automated analysis of 96-well plates with a throughput of more than 1,800 assay points-for a 23-plex assay-in 30 minutes. Researchers can increase the amount of information they obtain from rare or volume-limited samples, such as mouse or rat serum and decipher complex interrelationships among proteins involved in signal-transduction pathways. The user-friendly multiplex analysis system allows for simultaneous analysis of up to 100 different biomolecules (proteins, peptides, or nucleic acids) in a single microplate well. Offered as one-multiplex and two-singleplex assays designed to achieve favorable working-assay ranges, the assay delivers accurate and reproducible multiplex measurements of human-diabetes and obesity markers. Partial blocking of the antibody molecules on the bead surface was shown to reduce critical shear stress necessary to remove beads from the specific antigens, indicating that multiple antigen-antibody bonds held the beads on the array surface.Bio-Rad Laboratories Inc, Hercules, Calif, a multinational manufacturer and distributor of life science research and clinical-diagnostic products, has introduced the new Bio-Plex Pro™ human diabetes assay panels for its Bio-Plex® suspension-array system. The effect of cross-reactivity in the assay with magnetic beads detection could be decreased substantially by placing the array into a flow cell and subjecting the tethered beads to increasing shear flow, which removed beads first from the weakest cross-reactive antigens and then from more strong ones. It was demonstrated that the magnetic beads bound only those albumin spots to which antibody was reactive or cross-reactive in enzyme-linked immunosorbent assay (ELISA). Microarrays containing spots of nine albumins from sera of different mammals (human, bovine, sheep, goat, pig, dog, rabbit, rat, and mouse) were tested for their interaction with magnetic beads functionalized with monoclonal antibodies against bovine or human serum albumin. Here we addressed the problem of specificity and cross-reactivity in such detection, which (unlike conventional immunoassay methods) cannot rely on a difference in the equilibrium binding constants to distinguish between closely related antigens. Functionalized magnetic beads have been suggested recently as active labels for extremely rapid and highly sensitive immunoassay.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |